Title |
Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties
|
---|---|
Published in |
PLOS ONE, October 2013
|
DOI | 10.1371/journal.pone.0076751 |
Pubmed ID | |
Authors |
William A. Braff, Dana Willner, Philip Hugenholtz, Korneel Rabaey, Cullen R. Buie |
Abstract |
Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research. |
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Mendeley readers
Geographical breakdown
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Unknown | 82 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 21 | 25% |
Researcher | 11 | 13% |
Student > Master | 10 | 12% |
Student > Doctoral Student | 6 | 7% |
Professor | 6 | 7% |
Other | 14 | 17% |
Unknown | 15 | 18% |
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Environmental Science | 3 | 4% |
Other | 16 | 19% |
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