Title |
Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties
|
---|---|
Published in |
PLOS ONE, October 2013
|
DOI | 10.1371/journal.pone.0076751 |
Pubmed ID | |
Authors |
William A. Braff, Dana Willner, Philip Hugenholtz, Korneel Rabaey, Cullen R. Buie |
Abstract |
Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
Unknown | 81 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 26% |
Researcher | 11 | 13% |
Student > Master | 10 | 12% |
Professor | 6 | 7% |
Student > Doctoral Student | 6 | 7% |
Other | 12 | 15% |
Unknown | 16 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 32 | 39% |
Agricultural and Biological Sciences | 6 | 7% |
Biochemistry, Genetics and Molecular Biology | 4 | 5% |
Chemical Engineering | 3 | 4% |
Medicine and Dentistry | 3 | 4% |
Other | 14 | 17% |
Unknown | 20 | 24% |