| Title |
SPECT/CT Imaging of hNIS -Expression after Intravenous Delivery of an Oncolytic Adenovirus and 131I
|
|---|---|
| Published in |
PLOS ONE, March 2012
|
| DOI | 10.1371/journal.pone.0032871 |
| Pubmed ID | |
| Authors |
Maria Rajecki, Mirkka Sarparanta, Tanja Hakkarainen, Mikko Tenhunen, Iulia Diaconu, Venla Kuhmonen, Kalevi Kairemo, Anna Kanerva, Anu J. Airaksinen, Akseli Hemminki |
| Abstract |
Oncolytic adenoviruses can be engineered for better tumor selectivity, gene delivery and be armed for imaging and concentrating radionuclides into tumors for synergistic oncolysis. We constructed Ad5/3-hTERT-hNIS where replication is controlled by hTERT-promoter. Ad5/3-hTERT-hNIS expresses hNIS for imaging of transgene expression and for treatment of infected tumors by radioiodine. Ad5/3-hTERT-hNIS efficiently killed prostate cancer cells and induced iodine uptake in vitro and in vivo after intratumoral virus administration. Survival of mice treated with intravenous Ad5/3-hTERT-hNIS significantly prolonged survival over mock or radioiodine only but the combination of virus with radioiodine was not more effective than virus alone. Temporal and spatial changes in hNIS-expression during therapy were detected with SPECT, demonstrating feasibility of evaluation of the combination therapy with hNIS-expressing adenoviruses and radioiodide. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Colombia | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 19% |
| Student > Ph. D. Student | 4 | 19% |
| Student > Bachelor | 3 | 14% |
| Student > Postgraduate | 3 | 14% |
| Student > Master | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 29% |
| Medicine and Dentistry | 4 | 19% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Computer Science | 2 | 10% |
| Chemistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 29% |