Title |
SPECT/CT Imaging of hNIS -Expression after Intravenous Delivery of an Oncolytic Adenovirus and 131I
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Published in |
PLOS ONE, March 2012
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DOI | 10.1371/journal.pone.0032871 |
Pubmed ID | |
Authors |
Maria Rajecki, Mirkka Sarparanta, Tanja Hakkarainen, Mikko Tenhunen, Iulia Diaconu, Venla Kuhmonen, Kalevi Kairemo, Anna Kanerva, Anu J. Airaksinen, Akseli Hemminki |
Abstract |
Oncolytic adenoviruses can be engineered for better tumor selectivity, gene delivery and be armed for imaging and concentrating radionuclides into tumors for synergistic oncolysis. We constructed Ad5/3-hTERT-hNIS where replication is controlled by hTERT-promoter. Ad5/3-hTERT-hNIS expresses hNIS for imaging of transgene expression and for treatment of infected tumors by radioiodine. Ad5/3-hTERT-hNIS efficiently killed prostate cancer cells and induced iodine uptake in vitro and in vivo after intratumoral virus administration. Survival of mice treated with intravenous Ad5/3-hTERT-hNIS significantly prolonged survival over mock or radioiodine only but the combination of virus with radioiodine was not more effective than virus alone. Temporal and spatial changes in hNIS-expression during therapy were detected with SPECT, demonstrating feasibility of evaluation of the combination therapy with hNIS-expressing adenoviruses and radioiodide. |
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