Unregistered Submission
No, it does not entirely resemble the native sag :http://jn.physiology.org/content/77/4/1813For the MP changes abruptly upon steps, and there is no tail upon its termination...
No, it does not entirely resemble the native sag :http://jn.physiology.org/content/77/4/1813For the MP changes abruptly upon steps, and there is no tail upon its termination...
If :“ sag ratio is calculated as the ratio of the PEAK hyperpolarization DIVIDED by the STEADY-STATE hyperpolarization for the negative current injection ”Then values should be at least 1 and more…
? Do HCN channels activate considerably during 30 ms- Sag is sag independent of neuronal type albeit the timing and amplitude- Then also the HCN KO has a symmetrical sag in Figure 1A as proved by 1B
That reference from Unreg is CA1 neurons. The model is of a stellate neuron in MEC. Compare to the data in Figure 3 here. http://www.jneurosci...
I agree with Unreg. The sag in fig 1 looks different than what I'm used to seeing (e.g fig 3 here: https://www.ncbi.nlm.nih.gov/pubmed/?term=15550252 )
These can not be considered as a sag, for 1 those are symmetrical deflections occurring also upon the depolarization2 native cells never exhibit sag upon mild steps3 there is no hyperpolarization at…
The "sag" shortly after the onset of the hyperpolarizing traces is the characteristic property of a membrane with HCN channel expression.
Is it about HCN or what ?Where is the hallmark that indicates the presence of HCN during MP responses in Figure 1A – WT ?