Title |
A Riboswitch-Based Inducible Gene Expression System for Mycobacteria
|
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Published in |
PLOS ONE, January 2012
|
DOI | 10.1371/journal.pone.0029266 |
Pubmed ID | |
Authors |
Jessica C. Seeliger, Shana Topp, Kimberly M. Sogi, Mary L. Previti, Justin P. Gallivan, Carolyn R. Bertozzi |
Abstract |
Research on the human pathogen Mycobacterium tuberculosis (Mtb) would benefit from novel tools for regulated gene expression. Here we describe the characterization and application of a synthetic riboswitch-based system, which comprises a mycobacterial promoter for transcriptional control and a riboswitch for translational control. The system was used to induce and repress heterologous protein overexpression reversibly, to create a conditional gene knockdown, and to control gene expression in a macrophage infection model. Unlike existing systems for controlling gene expression in Mtb, the riboswitch does not require the co-expression of any accessory proteins: all of the regulatory machinery is encoded by a short DNA segment directly upstream of the target gene. The inducible riboswitch platform has the potential to be a powerful general strategy for creating customized gene regulation systems in Mtb. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 2 | <1% |
United States | 2 | <1% |
United Kingdom | 1 | <1% |
Hungary | 1 | <1% |
Denmark | 1 | <1% |
Iran, Islamic Republic of | 1 | <1% |
Unknown | 201 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 53 | 25% |
Researcher | 45 | 22% |
Student > Master | 28 | 13% |
Student > Bachelor | 22 | 11% |
Student > Postgraduate | 10 | 5% |
Other | 24 | 11% |
Unknown | 27 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 77 | 37% |
Biochemistry, Genetics and Molecular Biology | 59 | 28% |
Chemistry | 14 | 7% |
Immunology and Microbiology | 10 | 5% |
Engineering | 7 | 3% |
Other | 14 | 7% |
Unknown | 28 | 13% |