Title |
Barcoding and Border Biosecurity: Identifying Cyprinid Fishes in the Aquarium Trade
|
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Published in |
PLOS ONE, January 2012
|
DOI | 10.1371/journal.pone.0028381 |
Pubmed ID | |
Authors |
Rupert A. Collins, Karen F. Armstrong, Rudolf Meier, Youguang Yi, Samuel D. J. Brown, Robert H. Cruickshank, Suzanne Keeling, Colin Johnston |
Abstract |
Poorly regulated international trade in ornamental fishes poses risks to both biodiversity and economic activity via invasive alien species and exotic pathogens. Border security officials need robust tools to confirm identifications, often requiring hard-to-obtain taxonomic literature and expertise. DNA barcoding offers a potentially attractive tool for quarantine inspection, but has yet to be scrutinised for aquarium fishes. Here, we present a barcoding approach for ornamental cyprinid fishes by: (1) expanding current barcode reference libraries; (2) assessing barcode congruence with morphological identifications under numerous scenarios (e.g. inclusion of GenBank data, presence of singleton species, choice of analytical method); and (3) providing supplementary information to identify difficult species. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 14% |
United States | 1 | 14% |
Germany | 1 | 14% |
Australia | 1 | 14% |
Unknown | 3 | 43% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 4 | 57% |
Members of the public | 3 | 43% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 2% |
India | 3 | 1% |
Spain | 2 | <1% |
Portugal | 2 | <1% |
Australia | 1 | <1% |
South Africa | 1 | <1% |
Indonesia | 1 | <1% |
France | 1 | <1% |
Canada | 1 | <1% |
Other | 6 | 3% |
Unknown | 205 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 49 | 22% |
Researcher | 42 | 19% |
Student > Master | 32 | 14% |
Student > Bachelor | 18 | 8% |
Professor > Associate Professor | 13 | 6% |
Other | 43 | 19% |
Unknown | 30 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 135 | 59% |
Biochemistry, Genetics and Molecular Biology | 21 | 9% |
Environmental Science | 12 | 5% |
Social Sciences | 5 | 2% |
Arts and Humanities | 3 | 1% |
Other | 16 | 7% |
Unknown | 35 | 15% |