Title |
Assessment of α-Synuclein Secretion in Mouse and Human Brain Parenchyma
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Published in |
PLOS ONE, July 2011
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DOI | 10.1371/journal.pone.0022225 |
Pubmed ID | |
Authors |
Evangelia Emmanouilidou, Dimitris Elenis, Themis Papasilekas, Georgios Stranjalis, Kyriaki Gerozissis, Penelopi C. Ioannou, Kostas Vekrellis |
Abstract |
Genetic, biochemical, and animal model studies strongly suggest a central role for α-synuclein in the pathogenesis of Parkinson's disease. α-synuclein lacks a signal peptide sequence and has thus been considered a cytosolic protein. Recent data has suggested that the protein may be released from cells via a non-classical secretory pathway and may therefore exert paracrine effects in the extracellular environment. However, proof that α-synuclein is actually secreted into the brain extracellular space in vivo has not been obtained. We developed a novel highly sensitive ELISA in conjugation with an in vivo microdialysis technique to measure α-synuclein in brain interstitial fluid. We show for the first time that α-synuclein is readily detected in the interstitial fluid of both α-synuclein transgenic mice and human patients with traumatic brain injury. Our data suggest that α-synuclein is physiologically secreted by neurons in vivo. This interstitial fluid pool of the protein may have a role in the propagation of synuclein pathology and progression of Parkinson's disease. |
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Student > Bachelor | 22 | 14% |
Researcher | 21 | 14% |
Student > Master | 16 | 11% |
Student > Postgraduate | 8 | 5% |
Other | 25 | 16% |
Unknown | 23 | 15% |
Readers by discipline | Count | As % |
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Other | 17 | 11% |
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