RT @Kevin_McKernan: We toyed around 5-methylC and 5-hydroxymethyl C It was poison to PCR at high concentrations but handy at under 10%. ht…
RT @Kevin_McKernan: We toyed around 5-methylC and 5-hydroxymethyl C It was poison to PCR at high concentrations but handy at under 10%. ht…
We toyed around 5-methylC and 5-hydroxymethyl C It was poison to PCR at high concentrations but handy at under 10%. https://t.co/omjGC5uMGy https://t.co/FAf6J0Yvu1
@bxv_genomics @gringene_bio Very interesting. https://t.co/omjGC5uMGy
RT @Kevin_McKernan: It was a fun exploration of expanded genetic codes. We started playing around with 6 base PCR where hydroxymethyCTP an…
It was a fun exploration of expanded genetic codes. We started playing around with 6 base PCR where hydroxymethyCTP and methylCTP were used in conjunction with enzymes that could be leveraged to eliminate PCR products to keep a clean lab. https://t.co/oW
@jhewitt123 @PeterMcCormack Mito genomics is much harder than it appears on the surface. NUMTs and heteroplasmy make the topic quite tricky. Could certainly be acetyl-aldehyde. https://t.co/omjGC5uMGy
@gummibear737 @MackayIM This can be done using RNA bases in DNA amplifications or methylated bases in PCR that don’t exist in your target. These altered nucleotides become targets for enzymatic digestion so no residual PCR products can end up in your front
@pjie2 @EricTopol @StevenSalzberg1 @Wikipedia @PNASNews @CincyChildrens @CincyKidsGenomX NUMTs and lab contamination are a huge issue. We built methods to address this. PacBio has the readlengths and accuracy to address the problem and dance around the NUM
We have developed a PCR method, coined Déjà vu PCR, that utilizes six nucleotides in PCR with two methyl specific... http://t.co/Gifr3yIKOO
Expanded Genetic Codes in Next Generation Sequencing Enable Decontamination and Mitochondrial Enrichment. http://t.co/UgIdPXR9vR