Title |
The Influenza Virus Protein PB1-F2 Interacts with IKKβ and Modulates NF-κB Signalling
|
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Published in |
PLOS ONE, May 2013
|
DOI | 10.1371/journal.pone.0063852 |
Pubmed ID | |
Authors |
Ana Luísa Reis, John W. McCauley |
Abstract |
PB1-F2, a protein encoded by a second open reading frame of the influenza virus RNA segment 2, has emerged as a modulator of lung inflammatory responses but the molecular mechanisms underlying this are only poorly understood. Here we show that PB1-F2 inhibits the activation of NF-κB dependent signalling pathways in luciferase reporter assays. PB1-F2 proteins from four different viruses interact with IKKβ in yeast two-hybrid assays and by co-immunoprecipitation. PB1-F2 expression did not inhibit IKKβ kinase activity or NF-κB translocation into the nucleus, but NF-κB binding to DNA was severely impaired in PB1-F2 transfected cells as assessed by Electrophoretic Mobility Shift Assay. Neither the N-terminal 57 amino acid truncated forms nor the C-terminus of PB1-F2 were able to inhibit NF-κB dependent signalling, indicating that the full length protein is necessary for the inhibition. |
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Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 60% |
Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
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Unknown | 48 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 13 | 27% |
Student > Ph. D. Student | 8 | 17% |
Student > Master | 8 | 17% |
Student > Bachelor | 6 | 13% |
Student > Doctoral Student | 2 | 4% |
Other | 5 | 10% |
Unknown | 6 | 13% |
Readers by discipline | Count | As % |
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Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 2% |
Other | 3 | 6% |
Unknown | 9 | 19% |