Title |
Differential and Site Specific Impact of B Cells in the Protective Immune Response to Mycobacterium tuberculosis in the Mouse
|
---|---|
Published in |
PLOS ONE, April 2013
|
DOI | 10.1371/journal.pone.0061681 |
Pubmed ID | |
Authors |
Egídio Torrado, Jeffrey J. Fountain, Richard T. Robinson, Cynthia A. Martino, John E. Pearl, Javier Rangel-Moreno, Michael Tighe, Robert Dunn, Andrea M. Cooper |
Abstract |
Cell-mediated immune responses are known to be critical for control of mycobacterial infections whereas the role of B cells and humoral immunity is unclear. B cells can modulate immune responses by secretion of immunoglobulin, production of cytokines and antigen-presentation. To define the impact of B cells in the absence of secreted immunoglobulin, we analyzed the progression of Mycobacterium tuberculosis (Mtb) infection in mice that have B cells but which lack secretory immunoglobulin (AID(-/-)µS(-/-)mice). AID(-/-)µS(-/-) mice accumulated a population of activated B cells in the lungs when infected and were more susceptible to aerosol Mtb when compared to wild type (C57BL/6) mice or indeed mice that totally lack B cells. The enhanced susceptibility of AID(-/-)µS(-/-) mice was not associated with defective T cell activation or expression of a type 1 immune response. While delivery of normal serum to AID(-/-)µS(-/-) mice did not reverse susceptibility, susceptibility in the spleen was dependent upon the presence of B cells and susceptibility in the lungs of AID(-/-)µS(-/-)mice was associated with elevated expression of the cytokines IL-6, GM-CSF, IL-10 and molecules made by alternatively activated macrophages. Blocking of IL-10 signaling resulted in reversal of susceptibility in the spleens and lungs of AID(-/-)µS(-/-) mice. These data support the hypothesis that B cells can modulate immunity to Mtb in an organ specific manner via the modulation of cytokine production and macrophage activation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
United States | 1 | 1% |
Unknown | 77 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 20 | 25% |
Student > Ph. D. Student | 20 | 25% |
Student > Master | 8 | 10% |
Student > Bachelor | 5 | 6% |
Student > Doctoral Student | 4 | 5% |
Other | 9 | 11% |
Unknown | 13 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 30% |
Immunology and Microbiology | 19 | 24% |
Biochemistry, Genetics and Molecular Biology | 7 | 9% |
Medicine and Dentistry | 6 | 8% |
Nursing and Health Professions | 2 | 3% |
Other | 5 | 6% |
Unknown | 16 | 20% |