Title |
Paleogenomics in a Temperate Environment: Shotgun Sequencing from an Extinct Mediterranean Caprine
|
---|---|
Published in |
PLOS ONE, May 2009
|
DOI | 10.1371/journal.pone.0005670 |
Pubmed ID | |
Authors |
Oscar Ramírez, Elena Gigli, Pere Bover, Josep Antoni Alcover, Jaume Bertranpetit, Jose Castresana, Carles Lalueza-Fox |
Abstract |
Numerous endemic mammals, including dwarf elephants, goats, hippos and deers, evolved in isolation in the Mediterranean islands during the Pliocene and Pleistocene. Most of them subsequently became extinct during the Holocene. Recently developed high-throughput sequencing technologies could provide a unique tool for retrieving genomic data from these extinct species, making it possible to study their evolutionary history and the genetic bases underlying their particular, sometimes unique, adaptations. METHODOLOGY/PRINCIPALS FINDINGS: A DNA extraction of a approximately 6,000 year-old bone sample from an extinct caprine (Myotragus balearicus) from the Balearic Islands in the Western Mediterranean, has been subjected to shotgun sequencing with the GS FLX 454 platform. Only 0.27% of the resulting sequences, identified from alignments with the cow genome and comprising 15,832 nucleotides, with an average length of 60 nucleotides, proved to be endogenous. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 5% |
Germany | 1 | 1% |
Turkey | 1 | 1% |
United Kingdom | 1 | 1% |
Chile | 1 | 1% |
Spain | 1 | 1% |
Mexico | 1 | 1% |
Unknown | 77 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 23 | 26% |
Student > Ph. D. Student | 17 | 20% |
Student > Postgraduate | 8 | 9% |
Student > Master | 8 | 9% |
Student > Bachelor | 7 | 8% |
Other | 20 | 23% |
Unknown | 4 | 5% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 47 | 54% |
Biochemistry, Genetics and Molecular Biology | 14 | 16% |
Environmental Science | 7 | 8% |
Earth and Planetary Sciences | 7 | 8% |
Medicine and Dentistry | 2 | 2% |
Other | 4 | 5% |
Unknown | 6 | 7% |