Title |
Characterization of Human γδ T Lymphocytes Infiltrating Primary Malignant Melanomas
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Published in |
PLOS ONE, November 2012
|
DOI | 10.1371/journal.pone.0049878 |
Pubmed ID | |
Authors |
Adriana Cordova, Francesca Toia, Carmela La Mendola, Valentina Orlando, Serena Meraviglia, Gaetana Rinaldi, Matilde Todaro, Giuseppe Cicero, Leonardo Zichichi, Paolo Li Donni, Nadia Caccamo, Giorgio Stassi, Francesco Dieli, Francesco Moschella |
Abstract |
T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that γδ T cells mediate antigen-specific killing of tumor cells, we studied the representation and the in vitro cytokine production and cytotoxic activity of tumor infiltrating γδ T cells from 74 patients with primary melanoma. We found that γδ T cells represent the major lymphocyte population infiltrating melanoma, and both Vδ1(+) and Vδ2(+) cells are involved. The majority of melanoma-infiltrating γδ cells showed effector memory and terminally-differentiated phenotypes and, accordingly, polyclonal γδ T cell lines obtained from tumor-infiltrating immune cells produced IFN-γ and TNF-α and were capable of killing melanoma cell lines in vitro. The cytotoxic capability of Vδ2 cell lines was further improved by pre-treatment of tumor target cells with zoledronate. Moreover, higher rate of γδ T cells isolation and percentages of Vδ2 cells correlate with early stage of development of melanoma and absence of metastasis. Altogether, our results suggest that a natural immune response mediated by γδ T lymphocytes may contribute to the immunosurveillance of melanoma. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 1% |
Russia | 1 | <1% |
Germany | 1 | <1% |
Unknown | 147 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 31 | 21% |
Student > Ph. D. Student | 19 | 13% |
Student > Master | 18 | 12% |
Student > Bachelor | 13 | 9% |
Other | 10 | 7% |
Other | 22 | 15% |
Unknown | 38 | 25% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 31 | 21% |
Agricultural and Biological Sciences | 29 | 19% |
Biochemistry, Genetics and Molecular Biology | 20 | 13% |
Medicine and Dentistry | 14 | 9% |
Unspecified | 8 | 5% |
Other | 8 | 5% |
Unknown | 41 | 27% |