Title |
RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica
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Published in |
PLOS ONE, August 2014
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DOI | 10.1371/journal.pone.0105578 |
Pubmed ID | |
Authors |
Albert Mayola, Oihane Irazoki, Ignacio A. Martínez, Dmitri Petrov, Filippo Menolascina, Roman Stocker, José A. Reyes-Darias, Tino Krell, Jordi Barbé, Susana Campoy |
Abstract |
The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium. |
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Geographical breakdown
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United States | 1 | 2% |
Germany | 1 | 2% |
Unknown | 52 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 11 | 20% |
Student > Ph. D. Student | 10 | 18% |
Student > Master | 7 | 13% |
Professor > Associate Professor | 4 | 7% |
Student > Bachelor | 4 | 7% |
Other | 11 | 20% |
Unknown | 8 | 15% |
Readers by discipline | Count | As % |
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Environmental Science | 1 | 2% |
Other | 5 | 9% |
Unknown | 12 | 22% |