Title |
De Novo Assembly of the Perennial Ryegrass Transcriptome Using an RNA-Seq Strategy
|
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Published in |
PLOS ONE, August 2014
|
DOI | 10.1371/journal.pone.0103567 |
Pubmed ID | |
Authors |
Jacqueline D. Farrell, Stephen Byrne, Cristiana Paina, Torben Asp |
Abstract |
Perennial ryegrass is a highly heterozygous outbreeding grass species used for turf and forage production. Heterozygosity can affect de-Bruijn graph assembly making de novo transcriptome assembly of species such as perennial ryegrass challenging. Creating a reference transcriptome from a homozygous perennial ryegrass genotype can circumvent the challenge of heterozygosity. The goals of this study were to perform RNA-sequencing on multiple tissues from a highly inbred genotype to develop a reference transcriptome. This was complemented with RNA-sequencing of a highly heterozygous genotype for SNP calling. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 25% |
India | 1 | 13% |
France | 1 | 13% |
Unknown | 4 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 50% |
Scientists | 4 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Norway | 1 | 1% |
Brazil | 1 | 1% |
Canada | 1 | 1% |
New Zealand | 1 | 1% |
Belgium | 1 | 1% |
Spain | 1 | 1% |
Unknown | 69 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 19 | 25% |
Researcher | 16 | 21% |
Student > Master | 14 | 19% |
Student > Postgraduate | 6 | 8% |
Student > Doctoral Student | 5 | 7% |
Other | 7 | 9% |
Unknown | 8 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 50 | 67% |
Biochemistry, Genetics and Molecular Biology | 14 | 19% |
Environmental Science | 1 | 1% |
Nursing and Health Professions | 1 | 1% |
Medicine and Dentistry | 1 | 1% |
Other | 0 | 0% |
Unknown | 8 | 11% |