| Title |
The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype
|
|---|---|
| Published in |
PLOS ONE, June 2014
|
| DOI | 10.1371/journal.pone.0100268 |
| Pubmed ID | |
| Authors |
Sheran H. W. Law, Thomas D. Sargent |
| Abstract |
TALEN-based inactivation of the zebrafish pak4 gene resulted in embryos and adult fish that appear normal and fertile. This is in contrast to our previously published studies which were based on the use of antisense morpholino oligonucleotides (MOs). We have excluded potential explanations such as gene duplication, alternate splicing, cryptic initiation of translation, and translation-independent RNA function. Our conclusion is that pak4 is dispensable in zebrafish, and that even when corroborated by robust controls, such as RNA rescue, MOs may elicit misleading pseudophenotypes that do not correspond to results obtained by genetic mutations, and should thus be used with caution. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 3% |
| United Kingdom | 2 | 3% |
| Brazil | 1 | 2% |
| Austria | 1 | 2% |
| South Africa | 1 | 2% |
| Unknown | 56 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 22% |
| Student > Ph. D. Student | 12 | 19% |
| Student > Master | 9 | 14% |
| Student > Bachelor | 5 | 8% |
| Professor > Associate Professor | 4 | 6% |
| Other | 10 | 16% |
| Unknown | 9 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 23 | 37% |
| Agricultural and Biological Sciences | 22 | 35% |
| Medicine and Dentistry | 6 | 10% |
| Computer Science | 1 | 2% |
| Social Sciences | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 9 | 14% |