Title |
Rapid Development of Microsatellite Markers for Callosobruchus chinensis Using Illumina Paired-End Sequencing
|
---|---|
Published in |
PLOS ONE, May 2014
|
DOI | 10.1371/journal.pone.0095458 |
Pubmed ID | |
Authors |
Can-xing Duan, Dan-dan Li, Su-li Sun, Xiao-ming Wang, Zhen-dong Zhu |
Abstract |
The adzuki bean weevil, Callosobruchus chinensis L., is one of the most destructive pests of stored legume seeds such as mungbean, cowpea, and adzuki bean, which usually cause considerable loss in the quantity and quality of stored seeds during transportation and storage. However, a lack of genetic information of this pest results in a series of genetic questions remain largely unknown, including population genetic structure, kinship, biotype abundance, and so on. Co-dominant microsatellite markers offer a great resolving power to determine these events. Here, we report rapid microsatellite isolation from C. chinensis via high-throughput sequencing. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Germany | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Malaysia | 1 | 2% |
Chile | 1 | 2% |
United States | 1 | 2% |
Czechia | 1 | 2% |
Unknown | 44 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 11 | 23% |
Student > Ph. D. Student | 10 | 21% |
Researcher | 8 | 17% |
Student > Bachelor | 4 | 8% |
Student > Doctoral Student | 3 | 6% |
Other | 6 | 13% |
Unknown | 6 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 65% |
Biochemistry, Genetics and Molecular Biology | 4 | 8% |
Neuroscience | 2 | 4% |
Mathematics | 1 | 2% |
Unspecified | 1 | 2% |
Other | 2 | 4% |
Unknown | 7 | 15% |