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Rapid Development of Microsatellite Markers for Callosobruchus chinensis Using Illumina Paired-End Sequencing

Overview of attention for article published in PLOS ONE, May 2014
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Title
Rapid Development of Microsatellite Markers for Callosobruchus chinensis Using Illumina Paired-End Sequencing
Published in
PLOS ONE, May 2014
DOI 10.1371/journal.pone.0095458
Pubmed ID
Authors

Can-xing Duan, Dan-dan Li, Su-li Sun, Xiao-ming Wang, Zhen-dong Zhu

Abstract

The adzuki bean weevil, Callosobruchus chinensis L., is one of the most destructive pests of stored legume seeds such as mungbean, cowpea, and adzuki bean, which usually cause considerable loss in the quantity and quality of stored seeds during transportation and storage. However, a lack of genetic information of this pest results in a series of genetic questions remain largely unknown, including population genetic structure, kinship, biotype abundance, and so on. Co-dominant microsatellite markers offer a great resolving power to determine these events. Here, we report rapid microsatellite isolation from C. chinensis via high-throughput sequencing.

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Geographical breakdown

Country Count As %
Malaysia 1 2%
Chile 1 2%
United States 1 2%
Czechia 1 2%
Unknown 44 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 11 23%
Student > Ph. D. Student 10 21%
Researcher 8 17%
Student > Bachelor 4 8%
Student > Doctoral Student 3 6%
Other 6 13%
Unknown 6 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 65%
Biochemistry, Genetics and Molecular Biology 4 8%
Neuroscience 2 4%
Mathematics 1 2%
Unspecified 1 2%
Other 2 4%
Unknown 7 15%