Title |
Ultrasensitive Electrochemical Immunoassay for Avian Influenza Subtype H5 Using Nanocomposite
|
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Published in |
PLOS ONE, April 2014
|
DOI | 10.1371/journal.pone.0094685 |
Pubmed ID | |
Authors |
Zhixun Xie, Jiaoling Huang, Sisi Luo, Zhiqin Xie, Liji Xie, Jiabo Liu, Yaoshan Pang, Xianwen Deng, Qing Fan |
Abstract |
We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in detection sensitivity compared to the immunosensor without GO-PAb-BSA. We designed a PAb labeling GO strategy and signal amplification procedure that allow ultrasensitive and selective detection of AIV H5. The established method responded to 2(-15) HA unit/50 µL H5, with a linear calibration range from 2(-15) to 2(-8) HA unit/50 µL. In summary, we demonstrated that the immunosenser has a high specificity and sensitivity for AIV H5, and the established assay could be potentially applied in the rapid detection of other pathogenic microorganisms. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 49 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 20% |
Researcher | 9 | 18% |
Student > Master | 7 | 14% |
Student > Bachelor | 5 | 10% |
Professor > Associate Professor | 3 | 6% |
Other | 6 | 12% |
Unknown | 9 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 9 | 18% |
Biochemistry, Genetics and Molecular Biology | 6 | 12% |
Materials Science | 6 | 12% |
Agricultural and Biological Sciences | 5 | 10% |
Engineering | 4 | 8% |
Other | 8 | 16% |
Unknown | 11 | 22% |