| Title |
Extended-Spectrum-Beta-Lactamases, AmpC Beta-Lactamases and Plasmid Mediated Quinolone Resistance in Klebsiella spp. from Companion Animals in Italy
|
|---|---|
| Published in |
PLOS ONE, March 2014
|
| DOI | 10.1371/journal.pone.0090564 |
| Pubmed ID | |
| Authors |
Valentina Donati, Fabiola Feltrin, Rene S. Hendriksen, Christina Aaby Svendsen, Gessica Cordaro, Aurora García-Fernández, Serena Lorenzetti, Raniero Lorenzetti, Antonio Battisti, Alessia Franco |
| Abstract |
We report the genetic characterization of 15 Klebsiella pneumoniae (KP) and 4 isolates of K. oxytoca (KO) from clinical cases in dogs and cats and showing extended-spectrum cephalosporin (ESC) resistance. Extended spectrum beta-lactamase (ESBL) and AmpC genes, plasmid-mediated quinolone resistance (PMQR) and co-resistances were investigated. Among KP isolates, ST101 clone was predominant (8/15, 53%), followed by ST15 (4/15, 27%). ST11 and ST340, belonging to Clonal Complex (CC)11, were detected in 2012 (3/15, 20%). MLST on KP isolates corresponded well with PFGE results, with 11 different PFGE patterns observed, including two clusters of two (ST340) and four (ST101) indistinguishable isolates, respectively. All isolates harbored at least one ESBL or AmpC gene, all carried on transferable plasmids (IncR, IncFII, IncI1, IncN), and 16/19 were positive for PMQR genes (qnr family or aac(6')-Ib-cr). The most frequent ESBL was CTX-M-15 (11/19, 58%), detected in all KP ST101, in one KP ST15 and in both KP ST340. blaCTX-M-15 was carried on IncR plasmids in all but one KP isolate. All KP ST15 isolates harbored different ESC resistance genes and different plasmids, and presented the non-transferable blaSHV-28 gene, in association with blaCTX-M-15, blaCTX-M-1 (on IncR, or on IncN), blaSHV-2a (on IncR) or blaCMY-2 genes (on IncI1). KO isolates were positive for blaCTX-M-9 gene (on IncHI2), or for the blaSHV-12 and blaDHA-1 genes (on IncL/M). They were all positive for qnr genes, and one also for the aac(6')-Ib-cr gene. All Klebsiella isolates showed multiresistance towards aminoglycosides, sulfonamides, tetracyclines, trimethoprim and amphenicols, mediated by strA/B, aadA2, aadB, ant (2")-Ia, aac(6')-Ib, sul, tet, dfr and cat genes in various combinations. The emergence in pets of multidrug-resistant Klebsiella with ESBL, AmpC and PMQR determinants, poses further and serious challenges in companion animal therapy and raise concerns for possible bi-directional transmission between pets and humans, especially at household level. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 104 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | <1% |
| Guatemala | 1 | <1% |
| South Africa | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 100 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 19 | 18% |
| Researcher | 14 | 13% |
| Student > Ph. D. Student | 11 | 11% |
| Student > Doctoral Student | 11 | 11% |
| Student > Bachelor | 5 | 5% |
| Other | 20 | 19% |
| Unknown | 24 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 18 | 17% |
| Agricultural and Biological Sciences | 16 | 15% |
| Veterinary Science and Veterinary Medicine | 13 | 13% |
| Biochemistry, Genetics and Molecular Biology | 12 | 12% |
| Immunology and Microbiology | 10 | 10% |
| Other | 10 | 10% |
| Unknown | 25 | 24% |