| Title |
Dermal Substitutes Support the Growth of Human Skin-Derived Mesenchymal Stromal Cells: Potential Tool for Skin Regeneration
|
|---|---|
| Published in |
PLOS ONE, February 2014
|
| DOI | 10.1371/journal.pone.0089542 |
| Pubmed ID | |
| Authors |
Talita da Silva Jeremias, Rafaela Grecco Machado, Silvia Beatriz Coutinho Visoni, Maurício José Pereima, Dilmar Francisco Leonardi, Andrea Gonçalves Trentin |
| Abstract |
New strategies for skin regeneration are needed in order to provide effective treatment for cutaneous wounds and disease. Mesenchymal stem cells (MSCs) are an attractive source of cells for tissue engineering because of their prolonged self-renewal capacity, multipotentiality, and ability to release active molecules important for tissue repair. In this paper, we show that human skin-derived mesenchymal stromal cells (SD-MSCs) display similar characteristics to the multipotent MSCs. We also evaluate their growth in a three-dimensional (3D) culture system with dermal substitutes (Integra and Pelnac). When cultured in monolayers, SD-MSCs expressed mesenchymal markers, such as CD105, Fibronectin, and α-SMA; and neural markers, such as Nestin and βIII-Tubulin; at transcriptional and/or protein level. Integra and Pelnac equally supported the adhesion, spread and growth of human SD-MSCs in 3D culture, maintaining the MSC characteristics and the expression of multilineage markers. Therefore, dermal substitutes support the growth of mesenchymal stromal cells from human skin, promising an effective tool for tissue engineering and regenerative technology. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 83 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 2% |
| Japan | 1 | 1% |
| United Kingdom | 1 | 1% |
| Unknown | 79 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 16 | 19% |
| Student > Bachelor | 14 | 17% |
| Student > Doctoral Student | 10 | 12% |
| Researcher | 9 | 11% |
| Student > Ph. D. Student | 7 | 8% |
| Other | 16 | 19% |
| Unknown | 11 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 29% |
| Medicine and Dentistry | 12 | 14% |
| Biochemistry, Genetics and Molecular Biology | 11 | 13% |
| Engineering | 10 | 12% |
| Immunology and Microbiology | 3 | 4% |
| Other | 10 | 12% |
| Unknown | 13 | 16% |