| Title |
Assembly of the Novel Five-Component Apicomplexan Multi-Aminoacyl-tRNA Synthetase Complex Is Driven by the Hybrid Scaffold Protein Tg-p43
|
|---|---|
| Published in |
PLOS ONE, February 2014
|
| DOI | 10.1371/journal.pone.0089487 |
| Pubmed ID | |
| Authors |
Jason M. van Rooyen, Jean-Benjamin Murat, Pierre-Mehdi Hammoudi, Sylvie Kieffer-Jaquinod, Yohann Coute, Amit Sharma, Hervé Pelloux, Hassan Belrhali, Mohamed-Ali Hakimi |
| Abstract |
In Toxoplasma gondii, as in other eukaryotes, a subset of the amino-acyl-tRNA synthetases are arranged into an abundant cytoplasmic multi-aminoacyl-tRNA synthetase (MARS) complex. Through a series of genetic pull-down assays, we have identified the enzymes of this complex as: methionyl-, glutaminyl-, glutamyl-, and tyrosyl-tRNA synthetases, and we show that the N-terminal GST-like domain of a partially disordered hybrid scaffold protein, Tg-p43, is sufficient for assembly of the intact complex. Our gel filtration studies revealed significant heterogeneity in the size and composition of isolated MARS complexes. By targeting the tyrosyl-tRNA synthetases subunit, which was found exclusively in the complete 1 MDa complex, we were able to directly visualize MARS particles in the electron microscope. Image analyses of the negative stain data revealed the observed heterogeneity and instability of these complexes to be driven by the intrinsic flexibility of the domain arrangements within the MARS complex. These studies provide unique insights into the assembly of these ubiquitous but poorly understood eukaryotic complexes. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 37% |
| Student > Master | 7 | 20% |
| Researcher | 5 | 14% |
| Student > Bachelor | 4 | 11% |
| Other | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 2 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 46% |
| Biochemistry, Genetics and Molecular Biology | 11 | 31% |
| Chemistry | 2 | 6% |
| Nursing and Health Professions | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 3 | 9% |