Title |
Imaging Dynamic Molecular Signaling by the Cdc42 GTPase within the Developing CNS
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Published in |
PLOS ONE, February 2014
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DOI | 10.1371/journal.pone.0088870 |
Pubmed ID | |
Authors |
Nima Sharifai, Hasitha Samarajeewa, Daichi Kamiyama, Tzyy-Chyn Deng, Maria Boulina, Akira Chiba |
Abstract |
Protein interactions underlie the complexity of neuronal function. Potential interactions between specific proteins in the brain are predicted from assays based on genetic interaction and/or biochemistry. Genetic interaction reveals endogenous, but not necessarily direct, interactions between the proteins. Biochemistry-based assays, on the other hand, demonstrate direct interactions between proteins, but often outside their native environment or without a subcellular context. We aimed to achieve the best of both approaches by visualizing protein interaction directly within the brain of a live animal. Here, we show a proof-of-principle experiment in which the Cdc42 GTPase associates with its alleged partner WASp within neurons during the time and space that coincide with the newly developing CNS. |
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