Title |
Structural Characterization of the Mechanosensitive Channel Candidate MCA2 from Arabidopsis thaliana
|
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Published in |
PLOS ONE, January 2014
|
DOI | 10.1371/journal.pone.0087724 |
Pubmed ID | |
Authors |
Hideki Shigematsu, Kazuko Iida, Masataka Nakano, Pratima Chaudhuri, Hidetoshi Iida, Kuniaki Nagayama |
Abstract |
Mechanosensing in plants is thought to be governed by sensory complexes containing a Ca²⁺-permeable, mechanosensitive channel. The plasma membrane protein MCA1 and its paralog MCA2 from Arabidopsis thaliana are involved in mechanical stress-induced Ca²⁺ influx and are thus considered as candidates for such channels or their regulators. Both MCA1 and MCA2 were functionally expressed in Sf9 cells using a baculovirus system in order to elucidate their molecular natures. Because of the abundance of protein in these cells, MCA2 was chosen for purification. Purified MCA2 in a detergent-solubilized state formed a tetramer, which was confirmed by chemical cross-linking. Single-particle analysis of cryo-electron microscope images was performed to depict the overall shape of the purified protein. The three-dimensional structure of MCA2 was reconstructed at a resolution of 26 Å from 5,500 particles and appears to comprise a small transmembrane region and large cytoplasmic region. |
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Mendeley readers
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Professor | 1 | 2% |
Other | 3 | 5% |
Unknown | 11 | 20% |
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Physics and Astronomy | 1 | 2% |
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