| Title |
SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
|
|---|---|
| Published in |
PLOS ONE, December 2013
|
| DOI | 10.1371/journal.pone.0083997 |
| Pubmed ID | |
| Authors |
Adam Sateriale, Nathan H. Roy, Christopher D. Huston |
| Abstract |
Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica. |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 5% |
| Unknown | 21 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 23% |
| Researcher | 5 | 23% |
| Student > Master | 4 | 18% |
| Student > Bachelor | 2 | 9% |
| Student > Doctoral Student | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 41% |
| Immunology and Microbiology | 2 | 9% |
| Medicine and Dentistry | 2 | 9% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Physics and Astronomy | 1 | 5% |
| Other | 4 | 18% |
| Unknown | 3 | 14% |