| Title |
A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex
|
|---|---|
| Published in |
PLOS ONE, October 2013
|
| DOI | 10.1371/journal.pone.0077021 |
| Pubmed ID | |
| Authors |
Ka-Ho Leung, Lihua Lu, Modi Wang, Tsun-Yin Mak, Daniel Shiu-Hin Chan, Fung-Kit Tang, Chung-Hang Leung, Hiu-Yee Kwan, Zhiling Yu, Dik-Lung Ma |
| Abstract |
We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| China | 1 | 3% |
| Unknown | 33 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 26% |
| Student > Master | 7 | 20% |
| Student > Postgraduate | 5 | 14% |
| Student > Ph. D. Student | 4 | 11% |
| Other | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 6 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 12 | 34% |
| Biochemistry, Genetics and Molecular Biology | 6 | 17% |
| Agricultural and Biological Sciences | 6 | 17% |
| Medicine and Dentistry | 2 | 6% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 7 | 20% |