| Title |
Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke
|
|---|---|
| Published in |
PLOS ONE, August 2013
|
| DOI | 10.1371/journal.pone.0071478 |
| Pubmed ID | |
| Authors |
Vivek J. Srinivasan, Emiri T. Mandeville, Anil Can, Francesco Blasi, Mihail Climov, Ali Daneshmand, Jeong Hyun Lee, Esther Yu, Harsha Radhakrishnan, Eng H. Lo, Sava Sakadžić, Katharina Eikermann-Haerter, Cenk Ayata |
| Abstract |
Progress in experimental stroke and translational medicine could be accelerated by high-resolution in vivo imaging of disease progression in the mouse cortex. Here, we introduce optical microscopic methods that monitor brain injury progression using intrinsic optical scattering properties of cortical tissue. A multi-parametric Optical Coherence Tomography (OCT) platform for longitudinal imaging of ischemic stroke in mice, through thinned-skull, reinforced cranial window surgical preparations, is described. In the acute stages, the spatiotemporal interplay between hemodynamics and cell viability, a key determinant of pathogenesis, was imaged. In acute stroke, microscopic biomarkers for eventual infarction, including capillary non-perfusion, cerebral blood flow deficiency, altered cellular scattering, and impaired autoregulation of cerebral blood flow, were quantified and correlated with histology. Additionally, longitudinal microscopy revealed remodeling and flow recovery after one week of chronic stroke. Intrinsic scattering properties serve as reporters of acute cellular and vascular injury and recovery in experimental stroke. Multi-parametric OCT represents a robust in vivo imaging platform to comprehensively investigate these properties. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 100 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 3% |
| Unknown | 97 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 25 | 25% |
| Student > Ph. D. Student | 20 | 20% |
| Professor > Associate Professor | 7 | 7% |
| Student > Doctoral Student | 7 | 7% |
| Student > Master | 5 | 5% |
| Other | 20 | 20% |
| Unknown | 16 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 22 | 22% |
| Medicine and Dentistry | 17 | 17% |
| Neuroscience | 10 | 10% |
| Physics and Astronomy | 8 | 8% |
| Agricultural and Biological Sciences | 8 | 8% |
| Other | 14 | 14% |
| Unknown | 21 | 21% |