| Title |
Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface
|
|---|---|
| Published in |
PLOS ONE, July 2013
|
| DOI | 10.1371/journal.pone.0069058 |
| Pubmed ID | |
| Authors |
Andrew C. Payne, Michael Andregg, Kent Kemmish, Mark Hamalainen, Charlotte Bowell, Andrew Bleloch, Nathan Klejwa, Wolfgang Lehrach, Ken Schatz, Heather Stark, Adam Marblestone, George Church, Christopher S. Own, William Andregg |
| Abstract |
We present "molecular threading", a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and fluorescence and electron microscopies are used to characterize the angular distribution, straightness, and reproducibility of stretched DNA deposited in arrays onto elastomeric surfaces and thin membranes. Molecular threading demonstrates high straightness and uniformity over length scales from nanometers to micrometers, and represents an alternative to existing DNA deposition and linearization methods. These results point towards scalable and high-throughput precision manipulation of single-molecule polymers. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 9% |
| Russia | 1 | 3% |
| Italy | 1 | 3% |
| Unknown | 30 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 34% |
| Researcher | 8 | 23% |
| Student > Bachelor | 4 | 11% |
| Student > Master | 4 | 11% |
| Other | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 2 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 26% |
| Chemistry | 5 | 14% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Engineering | 4 | 11% |
| Physics and Astronomy | 3 | 9% |
| Other | 8 | 23% |
| Unknown | 2 | 6% |