| Title |
Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
|
|---|---|
| Published in |
PLOS ONE, July 2013
|
| DOI | 10.1371/journal.pone.0068708 |
| Pubmed ID | |
| Authors |
Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Morgan L. Maeder, Prakriti Kaini, Jeffry D. Sander, J. Keith Joung, Randall T. Peterson, Jing-Ruey Joanna Yeh |
| Abstract |
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-induced mutations are heritable, with efficiencies of germline transmission reaching as high as 100%. In addition, we extend the power of the RGN system by showing that these nucleases can be used with single-stranded oligodeoxynucleotides (ssODNs) to create precise intended sequence modifications, including single nucleotide substitutions. Finally, we describe and validate simple strategies that improve the targeting range of RGNs from 1 in every 128 basepairs (bps) of random DNA sequence to 1 in every 8 bps. Together, these advances expand the utility of the CRISPR-Cas system in the zebrafish beyond somatic indel formation to heritable and precise genome modifications. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 17% |
| Canada | 1 | 17% |
| Ireland | 1 | 17% |
| United States | 1 | 17% |
| Unknown | 2 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Scientists | 2 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 678 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 1% |
| United Kingdom | 4 | <1% |
| Japan | 3 | <1% |
| China | 3 | <1% |
| France | 2 | <1% |
| Denmark | 2 | <1% |
| Germany | 2 | <1% |
| Portugal | 2 | <1% |
| Finland | 1 | <1% |
| Other | 6 | <1% |
| Unknown | 646 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 158 | 23% |
| Researcher | 121 | 18% |
| Student > Bachelor | 87 | 13% |
| Student > Master | 85 | 13% |
| Student > Doctoral Student | 32 | 5% |
| Other | 95 | 14% |
| Unknown | 100 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 320 | 47% |
| Biochemistry, Genetics and Molecular Biology | 159 | 23% |
| Medicine and Dentistry | 31 | 5% |
| Neuroscience | 18 | 3% |
| Engineering | 8 | 1% |
| Other | 36 | 5% |
| Unknown | 106 | 16% |