Title |
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid
|
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Published in |
PLOS ONE, June 2013
|
DOI | 10.1371/journal.pone.0067592 |
Pubmed ID | |
Authors |
Gabriel I. Parra, JoLynn Azure, Renate Fischer, Karin Bok, Carlos Sandoval-Jaime, Stanislav V. Sosnovtsev, Peter Sander, Kim Y. Green |
Abstract |
Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52-56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 4% |
Japan | 1 | 2% |
Unknown | 46 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 19 | 39% |
Student > Ph. D. Student | 11 | 22% |
Student > Master | 4 | 8% |
Other | 3 | 6% |
Student > Doctoral Student | 2 | 4% |
Other | 3 | 6% |
Unknown | 7 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 19 | 39% |
Biochemistry, Genetics and Molecular Biology | 7 | 14% |
Medicine and Dentistry | 4 | 8% |
Immunology and Microbiology | 3 | 6% |
Environmental Science | 1 | 2% |
Other | 4 | 8% |
Unknown | 11 | 22% |