Title |
A Novel Fully Automated Molecular Diagnostic System (AMDS) for Colorectal Cancer Mutation Detection
|
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Published in |
PLOS ONE, May 2013
|
DOI | 10.1371/journal.pone.0062989 |
Pubmed ID | |
Authors |
Shiro Kitano, Jamie Myers, Junko Nakamura, Akio Yamane, Mami Yamashita, Masato Nakayama, Yusuke Tsukahara, Hiroshi Ushida, Wanqing Liu, Mark J. Ratain, Masahiko Amano |
Abstract |
KRAS, BRAF and PIK3CA mutations are frequently observed in colorectal cancer (CRC). In particular, KRAS mutations are strong predictors for clinical outcomes of EGFR-targeted treatments such as cetuximab and panitumumab in metastatic colorectal cancer (mCRC). For mutation analysis, the current methods are time-consuming, and not readily available to all oncologists and pathologists. We have developed a novel, simple, sensitive and fully automated molecular diagnostic system (AMDS) for point of care testing (POCT). Here we report the results of a comparison study between AMDS and direct sequencing (DS) in the detection of KRAS, BRAF and PI3KCA somatic mutations. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 18% |
Researcher | 5 | 18% |
Student > Master | 5 | 18% |
Other | 4 | 14% |
Student > Bachelor | 2 | 7% |
Other | 4 | 14% |
Unknown | 3 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 16 | 57% |
Agricultural and Biological Sciences | 5 | 18% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Nursing and Health Professions | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 1 | 4% |
Unknown | 2 | 7% |