Title |
Rapid Diagnosis of Bloodstream Infections with PCR Followed by Mass Spectrometry
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Published in |
PLOS ONE, April 2013
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DOI | 10.1371/journal.pone.0062108 |
Pubmed ID | |
Authors |
Elena Jordana-Lluch, Heather E. Carolan, Montserrat Giménez, Rangarajan Sampath, David J. Ecker, M. Dolores Quesada, Josep M. Mòdol, Fernando Arméstar, Lawrence B. Blyn, Lendell L. Cummins, Vicente Ausina, Elisa Martró |
Abstract |
Achieving a rapid microbiological diagnosis is crucial for decreasing morbidity and mortality of patients with a bloodstream infection, as it leads to the administration of an appropriate empiric antimicrobial therapy. Molecular methods may offer a rapid alternative to conventional microbiological diagnosis involving blood culture. In this study, the performance of a new technology that uses broad-spectrum PCR coupled with mass spectrometry (PCR/ESI-MS) was evaluated for the detection of microorganisms directly from whole blood. A total of 247 whole blood samples and paired blood cultures were prospectively obtained from 175 patients with a suspicion of sepsis. Both sample types were analyzed using the PCR/ESI-MS technology, and the results were compared with those obtained by conventional identification methods. The overall agreement between conventional methods and PCR/ESI-MS performed in blood culture aliquots was 94.2% with 96.8% sensitivity and 98.5% specificity for the molecular method. When comparing conventional methods with PCR/ESI-MS performed in whole blood specimens, the overall agreement was 77.1% with 50% sensitivity and 93.8% specificity for the molecular method. Interestingly, the PCR/ESI-MS technology led to the additional identification of 13 pathogens that were not found by conventional methods. Using the PCR/ESI-MS technology the microbiological diagnosis of bloodstream infections could be anticipated in about half of the patients in our setting, including a small but significant proportion of patients newly diagnosed. Thus, this promising technology could be very useful for the rapid diagnosis of sepsis in combination with traditional methods. |
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Spain | 2 | 67% |
United Kingdom | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 65 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 13 | 20% |
Student > Master | 13 | 20% |
Student > Ph. D. Student | 8 | 12% |
Student > Doctoral Student | 4 | 6% |
Professor | 4 | 6% |
Other | 12 | 18% |
Unknown | 11 | 17% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 23 | 35% |
Agricultural and Biological Sciences | 12 | 18% |
Immunology and Microbiology | 8 | 12% |
Engineering | 4 | 6% |
Computer Science | 3 | 5% |
Other | 3 | 5% |
Unknown | 12 | 18% |