Title |
A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern
|
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Published in |
PLOS ONE, April 2013
|
DOI | 10.1371/journal.pone.0061630 |
Pubmed ID | |
Authors |
Ignacio A. Martínez, Susana Campoy, Meritxell Tort, Montserrat Llagostera, Dmitri Petrov |
Abstract |
Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several Salmonella enterica serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the S. Typhimurium cheV mutant were characterized. |
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Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 5 | 15% |
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Student > Postgraduate | 2 | 6% |
Other | 2 | 6% |
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Immunology and Microbiology | 2 | 6% |
Other | 3 | 9% |
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