Title |
Klebsiella Phage vB_KleM-RaK2 — A Giant Singleton Virus of the Family Myoviridae
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Published in |
PLOS ONE, April 2013
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DOI | 10.1371/journal.pone.0060717 |
Pubmed ID | |
Authors |
Eugenijus Šimoliūnas, Laura Kaliniene, Lidija Truncaitė, Aurelija Zajančkauskaitė, Juozas Staniulis, Algirdas Kaupinis, Marija Ger, Mindaugas Valius, Rolandas Meškys |
Abstract |
At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2) is the largest Klebsiella infecting myovirus genome sequenced to date. In total, 272 out of 534 RaK2 ORFs lack detectable database homologues. Based on the similarity to biologically defined proteins and/or MS/MS analysis, 117 of RaK2 ORFs were given a functional annotation, including 28 RaK2 ORFs coding for structural proteins that have no reliable homologues to annotated structural proteins in other organisms. The electron micrographs revealed elaborate spike-like structures on the tail fibers of Rak2, suggesting that this phage is an atypical myovirus. While head and tail proteins of RaK2 are mostly myoviridae-related, the bioinformatics analysis indicate that tail fibers/spikes of this phage are formed from podovirus-like peptides predominantly. Overall, these results provide evidence that bacteriophage RaK2 differs profoundly from previously studied viruses of the Myoviridae family. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
South Africa | 1 | 1% |
Unknown | 67 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 22% |
Researcher | 12 | 17% |
Student > Master | 10 | 14% |
Student > Bachelor | 6 | 9% |
Professor > Associate Professor | 3 | 4% |
Other | 10 | 14% |
Unknown | 13 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 30% |
Biochemistry, Genetics and Molecular Biology | 18 | 26% |
Environmental Science | 3 | 4% |
Veterinary Science and Veterinary Medicine | 2 | 3% |
Immunology and Microbiology | 2 | 3% |
Other | 7 | 10% |
Unknown | 16 | 23% |