| Title |
Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
|
|---|---|
| Published in |
PLOS ONE, March 2013
|
| DOI | 10.1371/journal.pone.0059131 |
| Pubmed ID | |
| Authors |
Eduarda Moita, Angel Gil-Izquierdo, Carla Sousa, Federico Ferreres, Luís R. Silva, Patrícia Valentão, Raúl Domínguez-Perles, Nieves Baenas, Paula B. Andrade |
| Abstract |
Oxidative stress and inflammation play important roles in disease development. This study intended to evaluate the anti-inflammatory and antioxidant potential of Echium plantagineum L. bee pollen to support its claimed health beneficial effects. The hydromethanol extract efficiently scavenged nitric oxide ((•)NO) although against superoxide (O2(•-)) it behaved as antioxidant at lower concentrations and as pro-oxidant at higher concentrations. The anti-inflammatory potential was evaluated in LPS-stimulated macrophages. The levels of (•)NO and L-citrulline decreased for all extract concentrations tested, while the levels of prostaglandins, their metabolites and isoprostanes, evaluated by UPLC-MS, decreased with low extract concentrations. So, E. plantagineum bee pollen extract can exert anti-inflammatory activity by reducing (•)NO and prostaglandins. The extract is able to scavenge the reactive species (•)NO and O2(•-) and reduce markers of oxidative stress in cells at low concentrations. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 100 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 1% |
| United States | 1 | 1% |
| Sweden | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 96 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 18 | 18% |
| Student > Ph. D. Student | 16 | 16% |
| Researcher | 15 | 15% |
| Student > Master | 15 | 15% |
| Student > Doctoral Student | 6 | 6% |
| Other | 7 | 7% |
| Unknown | 23 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 22% |
| Biochemistry, Genetics and Molecular Biology | 11 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 10 | 10% |
| Medicine and Dentistry | 8 | 8% |
| Chemistry | 6 | 6% |
| Other | 14 | 14% |
| Unknown | 29 | 29% |