Title |
Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract
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Published in |
PLOS ONE, March 2013
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DOI | 10.1371/journal.pone.0058021 |
Pubmed ID | |
Authors |
Sarah Korup, Janita Rietscher, Sébastien Calvignac-Spencer, Franziska Trusch, Jörg Hofmann, Ugo Moens, Igor Sauer, Sebastian Voigt, Rosa Schmuck, Bernhard Ehlers |
Abstract |
Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large and small T antigens and the 3 structural proteins VP1, VP2 and VP3. Phylogenetic analyses did not reveal a close relationship to any known human or animal polyomavirus. Investigation of organs, body fluids and excretions of diseased individuals and healthy subjects with both HPyV12-specific nested PCR and quantitative real-time PCR revealed additional virus-positive samples of resected liver, cecum and rectum tissues and a positive fecal sample. A capsomer-based IgG ELISA was established using the major capsid protein VP1 of HPyV12. Seroprevalences of 23% and 17%, respectively, were determined in sera from healthy adults and adolescents and a pediatric group of children. These data indicate that the virus naturally infects humans and that primary infection may already occur in childhood. |
X Demographics
Geographical breakdown
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United States | 2 | 67% |
Netherlands | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 67% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | <1% |
Germany | 1 | <1% |
Unknown | 107 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 18 | 17% |
Student > Bachelor | 18 | 17% |
Student > Ph. D. Student | 16 | 15% |
Researcher | 12 | 11% |
Student > Doctoral Student | 8 | 7% |
Other | 14 | 13% |
Unknown | 23 | 21% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 31 | 28% |
Biochemistry, Genetics and Molecular Biology | 19 | 17% |
Medicine and Dentistry | 13 | 12% |
Immunology and Microbiology | 7 | 6% |
Engineering | 3 | 3% |
Other | 7 | 6% |
Unknown | 29 | 27% |