Title |
miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development
|
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Published in |
PLOS ONE, February 2013
|
DOI | 10.1371/journal.pone.0057080 |
Pubmed ID | |
Authors |
Chunyao Wei, Elizabeth J. Thatcher, Abigail F. Olena, Diana J. Cha, Ana L. Perdigoto, Andrew F. Marshall, Bruce D. Carter, Kendal Broadie, James G. Patton |
Abstract |
SNAP-25 is a core component of the trimeric SNARE complex mediating vesicle exocytosis during membrane addition for neuronal growth, neuropeptide/growth factor secretion, and neurotransmitter release during synaptic transmission. Here, we report a novel microRNA mechanism of SNAP-25 regulation controlling motor neuron development, neurosecretion, synaptic activity, and movement in zebrafish. Loss of miR-153 causes overexpression of SNAP-25 and consequent hyperactive movement in early zebrafish embryos. Conversely, overexpression of miR-153 causes SNAP-25 down regulation resulting in near complete paralysis, mimicking the effects of treatment with Botulinum neurotoxin. miR-153-dependent changes in synaptic activity at the neuromuscular junction are consistent with the observed movement defects. Underlying the movement defects, perturbation of miR-153 function causes dramatic developmental changes in motor neuron patterning and branching. Together, our results indicate that precise control of SNAP-25 expression by miR-153 is critically important for proper neuronal patterning as well as neurotransmission. |
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Unknown | 1 | 50% |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
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France | 1 | 2% |
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Researcher | 11 | 17% |
Student > Master | 10 | 16% |
Student > Bachelor | 7 | 11% |
Professor > Associate Professor | 3 | 5% |
Other | 4 | 6% |
Unknown | 14 | 22% |
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Physics and Astronomy | 2 | 3% |
Other | 4 | 6% |
Unknown | 12 | 19% |