Title |
Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches
|
---|---|
Published in |
PLOS ONE, February 2013
|
DOI | 10.1371/journal.pone.0056854 |
Pubmed ID | |
Authors |
Zhenyu Shi, Anthony G. Wedd, Sally L. Gras |
Abstract |
The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration. |
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Mendeley readers
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