Title |
FCHSD1 and FCHSD2 Are Expressed in Hair Cell Stereocilia and Cuticular Plate and Regulate Actin Polymerization In Vitro
|
---|---|
Published in |
PLOS ONE, February 2013
|
DOI | 10.1371/journal.pone.0056516 |
Pubmed ID | |
Authors |
Huiren Cao, Xiaolei Yin, Yujie Cao, Yecheng Jin, Shan Wang, Yanhui Kong, Yuexing Chen, Jiangang Gao, Stefan Heller, Zhigang Xu |
Abstract |
Mammalian FCHSD1 and FCHSD2 are homologous proteins containing an amino-terminal F-BAR domain and two SH3 domains near their carboxyl-termini. We report here that FCHSD1 and FCHSD2 are expressed in mouse cochlear sensory hair cells. FCHSD1 mainly localizes to the cuticular plate, whereas FCHSD2 mainly localizes along the stereocilia in a punctuate pattern. Nervous Wreck (Nwk), the Drosophila ortholog of FCHSD1 and FCHSD2, has been shown to bind Wsp and play an important role in F-actin assembly. We show that, like its Drosophila counterpart, FCHSD2 interacts with WASP and N-WASP, the mammalian orthologs of Drosophila Wsp, and stimulates F-actin assembly in vitro. In contrast, FCHSD1 doesn't bind WASP or N-WASP, and can't stimulate F-actin assembly when tested in vitro. We found, however, that FCHSD1 binds via its F-BAR domain to the SH3 domain of Sorting Nexin 9 (SNX9), a well characterized BAR protein that has been shown to promote WASP-Arp2/3-dependent F-actin polymerization. FCHSD1 greatly enhances SNX9's WASP-Arp2/3-dependent F-actin polymerization activity. In hair cells, SNX9 was detected in the cuticular plate, where it colocalizes with FCHSD1. Our results suggest that FCHSD1 and FCHSD2 could modulate F-actin assembly or maintenance in hair cell stereocilia and cuticular plate. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 2% |
Poland | 1 | 2% |
Unknown | 46 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 25% |
Researcher | 7 | 15% |
Professor | 4 | 8% |
Student > Doctoral Student | 4 | 8% |
Professor > Associate Professor | 4 | 8% |
Other | 8 | 17% |
Unknown | 9 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 33% |
Biochemistry, Genetics and Molecular Biology | 10 | 21% |
Neuroscience | 4 | 8% |
Medicine and Dentistry | 2 | 4% |
Psychology | 1 | 2% |
Other | 3 | 6% |
Unknown | 12 | 25% |