| Title |
The Maternal Transcriptome of the Crustacean Parhyale hawaiensis Is Inherited Asymmetrically to Invariant Cell Lineages of the Ectoderm and Mesoderm
|
|---|---|
| Published in |
PLOS ONE, February 2013
|
| DOI | 10.1371/journal.pone.0056049 |
| Pubmed ID | |
| Authors |
Peter Nestorov, Florian Battke, Mitchell P. Levesque, Matthias Gerberding |
| Abstract |
The embryo of the crustacean Parhyale hawaiensis has a total, unequal and invariant early cleavage pattern. It specifies cell fates earlier than other arthropods, including Drosophila, as individual blastomeres of the 8-cell stage are allocated to the germ layers and the germline. Furthermore, the 8-cell stage is amenable to embryological manipulations. These unique features make Parhyale a suitable system for elucidating germ layer specification in arthropods. Since asymmetric localization of maternally provided RNA is a widespread mechanism to specify early cell fates, we asked whether this is also true for Parhyale. A candidate gene approach did not find RNAs that are asymmetrically distributed at the 8-cell stage. Therefore, we designed a high-density microarray from 9400 recently sequenced ESTs (1) to identify maternally provided RNAs and (2) to find RNAs that are differentially distributed among cells of the 8-cell stage. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| Switzerland | 1 | 2% |
| France | 1 | 2% |
| India | 1 | 2% |
| United Kingdom | 1 | 2% |
| Unknown | 46 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 27% |
| Student > Ph. D. Student | 10 | 20% |
| Student > Master | 7 | 14% |
| Unspecified | 6 | 12% |
| Student > Bachelor | 2 | 4% |
| Other | 9 | 18% |
| Unknown | 3 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 19 | 37% |
| Biochemistry, Genetics and Molecular Biology | 12 | 24% |
| Unspecified | 6 | 12% |
| Environmental Science | 5 | 10% |
| Social Sciences | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 6 | 12% |