Title |
RNA Interference against Discoidin Domain Receptor 2 Ameliorates Alcoholic Liver Disease in Rats
|
---|---|
Published in |
PLOS ONE, February 2013
|
DOI | 10.1371/journal.pone.0055860 |
Pubmed ID | |
Authors |
Zheng Luo, Huimin Liu, Xiaomeng Sun, Rong Guo, Ruibing Cui, Xiangxing Ma, Ming Yan |
Abstract |
Discoidin domain receptor 2 (DDR2) is involved in fibrotic disease. However, the exact pathogenic implications of the receptor in early alcoholic liver disease are still controversial. We constructed plasmid vectors encoding short-hairpin RNA against DDR2 to investigate its role in alcoholic liver disease in an immortalized rat hepatic stellate cell line, HSC-T6, and in rats by MTT, RT-PCR and western blot analyses; immunohistochemistry and electron microscopy. Alcohol-induced upregulation of DDR2 was associated with the expression of matrix metalloproteinase 2, the transforming growth factor β1 signaling pathway and tissue inhibitor of metalloproteinase 1; collagen deposition; and extracellular matrix remodeling. Inhibition of DDR2 decreased HSC-T6 cell proliferation and liver injury in rats with 10-week-induced alcoholic liver disease. DDR2 may have an important role in the pathogenesis of early-stage alcoholic liver disease. Silencing DDR2 may be effective in preventing early-stage alcoholic liver disease. |
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Scientists | 1 | 50% |
Mendeley readers
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Researcher | 3 | 15% |
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Professor | 1 | 5% |
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Other | 1 | 5% |
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Unknown | 7 | 35% |