Title |
Production of LacZ Inducible T Cell Hybridoma Specific for Human and Mouse gp10025–33 Peptides
|
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Published in |
PLOS ONE, February 2013
|
DOI | 10.1371/journal.pone.0055583 |
Pubmed ID | |
Authors |
Gal Cafri, Adi Sharbi-Yunger, Esther Tzehoval, Lea Eisenbach |
Abstract |
Identification and quantification of immunogenic peptides and tumor-derived epitopes presented on MHC-I molecules are essential for basic studies and vaccines generation. Although lymphocytes derived from transgenic mice can serve as sensitive detectors of processes of antigen presentation and recognition, they are not always available. The use of cell lines might be extremely useful. In this study, we generated a lacZ inducible CD8⁺ hybridoma (BUSA14) capable of recognizing both human and mouse gp100₂₅₋₃₃ melanoma antigens presented on dendritic and tumor cell lines. This hybridoma expresses a variety of membranal T cell markers and secretes IL-2 and TNFα. Thus, BUSA14 offers a quantifiable, cheap and straightforward tool for studying peptide presentation by MHC-I molecules on the cell surface. |
X Demographics
Geographical breakdown
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United States | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
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Unknown | 52 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 20 | 38% |
Student > Bachelor | 7 | 13% |
Student > Master | 6 | 12% |
Other | 4 | 8% |
Researcher | 2 | 4% |
Other | 4 | 8% |
Unknown | 9 | 17% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 17 | 33% |
Biochemistry, Genetics and Molecular Biology | 9 | 17% |
Immunology and Microbiology | 9 | 17% |
Chemistry | 2 | 4% |
Environmental Science | 1 | 2% |
Other | 3 | 6% |
Unknown | 11 | 21% |