Title |
Monolayer Stress Microscopy: Limitations, Artifacts, and Accuracy of Recovered Intercellular Stresses
|
---|---|
Published in |
PLOS ONE, February 2013
|
DOI | 10.1371/journal.pone.0055172 |
Pubmed ID | |
Authors |
Dhananjay T. Tambe, Ugo Croutelle, Xavier Trepat, Chan Young Park, Jae Hun Kim, Emil Millet, James P. Butler, Jeffrey J. Fredberg |
Abstract |
In wound healing, tissue growth, and certain cancers, the epithelial or the endothelial monolayer sheet expands. Within the expanding monolayer sheet, migration of the individual cell is strongly guided by physical forces imposed by adjacent cells. This process is called plithotaxis and was discovered using Monolayer Stress Microscopy (MSM). MSM rests upon certain simplifying assumptions, however, concerning boundary conditions, cell material properties and system dimensionality. To assess the validity of these assumptions and to quantify associated errors, here we report new analytical, numerical, and experimental investigations. For several commonly used experimental monolayer systems, the simplifying assumptions used previously lead to errors that are shown to be quite small. Out-of-plane components of displacement and traction fields can be safely neglected, and characteristic features of intercellular stresses that underlie plithotaxis remain largely unaffected. Taken together, these findings validate Monolayer Stress Microscopy within broad but well-defined limits of applicability. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 2% |
France | 3 | 1% |
Italy | 1 | <1% |
Netherlands | 1 | <1% |
Denmark | 1 | <1% |
United Kingdom | 1 | <1% |
Japan | 1 | <1% |
Spain | 1 | <1% |
Unknown | 203 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 66 | 31% |
Researcher | 33 | 15% |
Student > Master | 27 | 13% |
Student > Doctoral Student | 15 | 7% |
Student > Bachelor | 13 | 6% |
Other | 34 | 16% |
Unknown | 28 | 13% |
Readers by discipline | Count | As % |
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Engineering | 57 | 26% |
Agricultural and Biological Sciences | 39 | 18% |
Physics and Astronomy | 27 | 13% |
Biochemistry, Genetics and Molecular Biology | 23 | 11% |
Materials Science | 7 | 3% |
Other | 26 | 12% |
Unknown | 37 | 17% |