| Title |
Early Detection of Abnormal Prion Protein in Genetic Human Prion Diseases Now Possible Using Real-Time QUIC Assay
|
|---|---|
| Published in |
PLOS ONE, January 2013
|
| DOI | 10.1371/journal.pone.0054915 |
| Pubmed ID | |
| Authors |
Kazunori Sano, Katsuya Satoh, Ryuichiro Atarashi, Hiroshi Takashima, Yasushi Iwasaki, Mari Yoshida, Nobuo Sanjo, Hiroyuki Murai, Hidehiro Mizusawa, Matthias Schmitz, Inga Zerr, Yong-Sun Kim, Noriyuki Nishida |
| Abstract |
The definitive diagnosis of genetic prion diseases (gPrD) requires pathological confirmation. To date, diagnosis has relied upon the finding of the biomarkers 14-3-3 protein and total tau (t-tau) protein in the cerebrospinal fluid (CSF), but many researchers have reported that these markers are not sufficiently elevated in gPrD, especially in Gerstmann-Sträussler-Scheinker syndrome (GSS). We recently developed a new in vitro amplification technology, designated "real-time quaking-induced conversion (RT-QUIC)", to detect the abnormal form of prion protein in CSF from sporadic Creutzfeldt-Jakob disease (sCJD) patients. In the present study, we aimed to investigate the presence of biomarkers and evaluate RT-QUIC assay in patients with gPrD, as the utility of RT-QUIC as a diagnostic tool in gPrD has yet to be determined. |
Mendeley readers
The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Greece | 1 | <1% |
| Germany | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 98 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 20% |
| Researcher | 19 | 19% |
| Student > Bachelor | 13 | 13% |
| Professor | 7 | 7% |
| Student > Master | 7 | 7% |
| Other | 17 | 17% |
| Unknown | 19 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 20 | 20% |
| Agricultural and Biological Sciences | 18 | 18% |
| Biochemistry, Genetics and Molecular Biology | 15 | 15% |
| Neuroscience | 13 | 13% |
| Immunology and Microbiology | 3 | 3% |
| Other | 9 | 9% |
| Unknown | 24 | 24% |