| Title |
Transgenic Fluorescent Plasmodium cynomolgi Liver Stages Enable Live Imaging and Purification of Malaria Hypnozoite-Forms
|
|---|---|
| Published in |
PLOS ONE, January 2013
|
| DOI | 10.1371/journal.pone.0054888 |
| Pubmed ID | |
| Authors |
Annemarie Voorberg-van der Wel, Anne-Marie Zeeman, Sandra M. van Amsterdam, Alexander van den Berg, Els J. Klooster, Shiroh Iwanaga, Chris J. Janse, Geert-Jan van Gemert, Robert Sauerwein, Niels Beenhakker, Gerrit Koopman, Alan W. Thomas, Clemens H. M. Kocken |
| Abstract |
A major challenge for strategies to combat the human malaria parasite Plasmodium vivax is the presence of hypnozoites in the liver. These dormant forms can cause renewed clinical disease after reactivation through unknown mechanisms. The closely related non-human primate malaria P. cynomolgi is a frequently used model for studying hypnozoite-induced relapses. Here we report the generation of the first transgenic P. cynomolgi parasites that stably express fluorescent markers in liver stages by transfection with novel DNA-constructs containing a P. cynomolgi centromere. Analysis of fluorescent liver stages in culture identified, in addition to developing liver-schizonts, uninucleate persisting parasites that were atovaquone resistant but primaquine sensitive, features associated with hypnozoites. We demonstrate that these hypnozoite-forms could be isolated by fluorescence-activated cell sorting. The fluorescently-tagged parasites in combination with FACS-purification open new avenues for a wide range of studies for analysing hypnozoite biology and reactivation. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 1% |
| Indonesia | 1 | 1% |
| Brazil | 1 | 1% |
| India | 1 | 1% |
| United Kingdom | 1 | 1% |
| Unknown | 65 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 25 | 36% |
| Student > Ph. D. Student | 13 | 19% |
| Other | 4 | 6% |
| Lecturer | 4 | 6% |
| Student > Master | 4 | 6% |
| Other | 8 | 11% |
| Unknown | 12 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 37% |
| Biochemistry, Genetics and Molecular Biology | 9 | 13% |
| Medicine and Dentistry | 9 | 13% |
| Immunology and Microbiology | 7 | 10% |
| Social Sciences | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 13 | 19% |