Title |
P-Rex1 Cooperates with PDGFRβ to Drive Cellular Migration in 3D Microenvironments
|
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Published in |
PLOS ONE, January 2013
|
DOI | 10.1371/journal.pone.0053982 |
Pubmed ID | |
Authors |
Andrew D. Campbell, Samuel Lawn, Lynn C. McGarry, Heidi C. Welch, Bradford W. Ozanne, Jim C. Norman |
Abstract |
Expression of the Rac-guanine nucleotide exchange factor (RacGEF), P-Rex1 is a key determinant of progression to metastasis in a number of human cancers. In accordance with this proposed role in cancer cell invasion and metastasis, we find that ectopic expression of P-Rex1 in an immortalised human fibroblast cell line is sufficient to drive multiple migratory and invasive phenotypes. The invasive phenotype is greatly enhanced by the presence of a gradient of serum or platelet-derived growth factor, and is dependent upon the expression of functional PDGF receptor β. Consistently, the invasiveness of WM852 melanoma cells, which endogenously express P-Rex1 and PDGFRβ, is opposed by siRNA of either of these proteins. Furthermore, the current model of P-Rex1 activation is advanced through demonstration of P-Rex1 and PDGFRβ as components of the same macromolecular complex. These data suggest that P-Rex1 has an influence on physiological migratory processes, such as invasion of cancer cells, both through effects upon classical Rac1-driven motility and a novel association with RTK signalling complexes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 31 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 39% |
Researcher | 6 | 19% |
Student > Master | 4 | 13% |
Student > Bachelor | 2 | 6% |
Professor > Associate Professor | 2 | 6% |
Other | 1 | 3% |
Unknown | 4 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 45% |
Biochemistry, Genetics and Molecular Biology | 7 | 23% |
Engineering | 2 | 6% |
Immunology and Microbiology | 1 | 3% |
Nursing and Health Professions | 1 | 3% |
Other | 2 | 6% |
Unknown | 4 | 13% |