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Quinacrine Impairs Enterovirus 71 RNA Replication by Preventing Binding of Polypyrimidine-Tract Binding Protein with Internal Ribosome Entry Sites

Overview of attention for article published in PLOS ONE, January 2013
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Title
Quinacrine Impairs Enterovirus 71 RNA Replication by Preventing Binding of Polypyrimidine-Tract Binding Protein with Internal Ribosome Entry Sites
Published in
PLOS ONE, January 2013
DOI 10.1371/journal.pone.0052954
Pubmed ID
Authors

Jianmin Wang, Jiang Du, Zhiqiang Wu, Qi Jin

Abstract

Since the 1980s, epidemics of enterovirus 71 (EV71) and other enteroviruses have occurred in Asian countries and regions, causing a wide range of human diseases. No effective therapy is available for the treatment of these infections. Internal ribosome entry sites (IRESs) are indispensable for the initiation of translation in enteroviruses. Several cellular factors, as well as the ribosome, are recruited to the conserved IRES during this process. Quinacrine intercalates into the RNA architecture and inhibits RNA transcription and protein synthesis, and a recent study showed that quinacrine inhibited encephalomyocarditis virus and poliovirus IRES-mediated translation in vitro without disrupting internal cellular IRES. Here, we report that quinacrine was highly active against EV71, protecting cells from EV71 infection. Replication of viral RNA, expression of viral capsid protein, and production of virus were all strongly inhibited by quinacrine. Interaction of the polypyrimidine tract-binding protein (PTB) with the conserved IRES was prevented by quinacrine. Coxsackieviruses and echovirus were also inhibited by quinacrine in cultured cells. These results indicate that quinacrine may serve as a potential protective agent for use in the treatment of patients with chronic enterovirus infection.

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Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 14%
Student > Doctoral Student 4 14%
Researcher 4 14%
Student > Master 4 14%
Student > Postgraduate 3 10%
Other 6 21%
Unknown 4 14%
Readers by discipline Count As %
Medicine and Dentistry 8 28%
Agricultural and Biological Sciences 6 21%
Biochemistry, Genetics and Molecular Biology 5 17%
Chemistry 2 7%
Unspecified 1 3%
Other 2 7%
Unknown 5 17%