| Title |
The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth
|
|---|---|
| Published in |
PLOS ONE, December 2012
|
| DOI | 10.1371/journal.pone.0051540 |
| Pubmed ID | |
| Authors |
Vladimir Yong-Gonzales, Lisa E. Hang, Federica Castellucci, Dana Branzei, Xiaolan Zhao |
| Abstract |
The Smc5-Smc6 complex in Saccharomyces cerevisiae is both essential for growth and important for coping with genotoxic stress. While it facilitates damage tolerance throughout the genome under genotoxin treatment, its function during unperturbed growth is mainly documented for repetitive DNA sequence maintenance. Here we provide physical and genetic evidence showing that the Smc5-Smc6 complex regulates recombination at non-repetitive loci such as centromeres in the absence of DNA damaging agents. Mutating Smc6 results in the accumulation of recombination intermediates at centromeres and other unique sequences as assayed by 2D gel analysis. In addition, smc6 mutant cells exhibit increased levels of Rad52 foci that co-localize with centromere markers. A rad52 mutation that decreases centromeric, but not overall, levels of Rad52 foci in smc6 mutants suppresses the nocodazole sensitivity of these cells, suggesting that the Smc6-mediated regulation of recombination at centromeric regions impacts centromere-related functions. In addition to influencing recombination, the SUMO ligase subunit of the Smc5-Smc6 complex promotes the sumoylation of two kinetochore proteins and affects mitotic spindles. These results suggest that the Smc5-Smc6 complex regulates both recombination and kinetochore sumoylation to facilitate chromosomal maintenance during growth. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 53 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 30% |
| Student > Ph. D. Student | 15 | 28% |
| Student > Bachelor | 5 | 9% |
| Student > Master | 5 | 9% |
| Student > Doctoral Student | 4 | 8% |
| Other | 4 | 8% |
| Unknown | 4 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 45% |
| Biochemistry, Genetics and Molecular Biology | 20 | 38% |
| Medicine and Dentistry | 2 | 4% |
| Immunology and Microbiology | 1 | 2% |
| Earth and Planetary Sciences | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 4 | 8% |