| Title |
Evaluation of Peripheral Blood Mononuclear Cell Processing and Analysis for Survival Motor Neuron Protein
|
|---|---|
| Published in |
PLOS ONE, November 2012
|
| DOI | 10.1371/journal.pone.0050763 |
| Pubmed ID | |
| Authors |
Dione T. Kobayashi, Douglas Decker, Phillip Zaworski, Karen Klott, Julie McGonigal, Nabil Ghazal, Laurel Sly, Brett Chung, James Vanderlugt, Karen S. Chen |
| Abstract |
Survival Motor Neuron (SMN) protein levels may become key pharmacodynamic (PD) markers in spinal muscular atrophy (SMA) clinical trials. SMN protein in peripheral blood mononuclear cells (PBMCs) can be quantified for trials using an enzyme-linked immunosorbent assay (ELISA). We developed protocols to collect, process, store and analyze these samples in a standardized manner for SMA clinical studies, and to understand the impact of age and intraindividual variability over time on PBMC SMN signal. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Egypt | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Burkina Faso | 1 | 2% |
| Unknown | 40 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 29% |
| Researcher | 6 | 15% |
| Student > Bachelor | 6 | 15% |
| Student > Master | 5 | 12% |
| Other | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 7 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 27% |
| Agricultural and Biological Sciences | 9 | 22% |
| Medicine and Dentistry | 6 | 15% |
| Neuroscience | 4 | 10% |
| Nursing and Health Professions | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 6 | 15% |