| Title |
Performance of LBSap Vaccine after Intradermal Challenge with L. infantum and Saliva of Lu. longipalpis: Immunogenicity and Parasitological Evaluation
|
|---|---|
| Published in |
PLOS ONE, November 2012
|
| DOI | 10.1371/journal.pone.0049780 |
| Pubmed ID | |
| Authors |
Bruno Mendes Roatt, Rodrigo Dian de Oliveira Aguiar-Soares, Juliana Vitoriano-Souza, Wendel Coura-Vital, Samuel Leôncio Braga, Rodrigo Corrêa-Oliveira, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho, Marta de Lana, Nelder Figueiredo Gontijo, Marcos José Marques, Rodolfo Cordeiro Giunchetti, Alexandre Barbosa Reis |
| Abstract |
In the last decade, the search for new vaccines against canine visceral leishmaniasis has intensified. However, the pattern related to immune protection during long periods after experimental infection in vaccine trials is still not fully understood. Herein, we investigated the immunogenicity and parasitological levels after intradermal challenge with Leishmania infantum plus salivary gland extract in dogs immunized with a vaccine composed of L. braziliensis antigens plus saponin as an adjuvant (LBSap vaccine). The LBSap vaccine elicited higher levels of total anti-Leishmania IgG as well as both IgG1 and IgG2. Furthermore, dogs vaccinated had increased levels of lymphocytes, particularly circulating B cells (CD21(+)) and both CD4(+) and CD8(+) T lymphocytes. LBSap also elicited an intense in vitro cell proliferation associated with higher levels of CD4(+) T lymphocytes specific for vaccine soluble antigen and soluble lysate of L. infantum antigen even 885 days after experimental challenge. Furthermore, LBSap vaccinated dogs presented high IFN-γ and low IL-10 and TGF-β1 expression in spleen with significant reduction of parasite load in this tissue. Overall, our results validate the potential of LBSap vaccine to protect against L. infantum experimental infection and strongly support further evaluation of efficiency of LBSap against CVL in natural infection conditions. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | 2% |
| India | 1 | 1% |
| France | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 76 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 22% |
| Student > Master | 16 | 20% |
| Student > Ph. D. Student | 9 | 11% |
| Student > Bachelor | 8 | 10% |
| Student > Postgraduate | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 19 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 27% |
| Veterinary Science and Veterinary Medicine | 10 | 12% |
| Immunology and Microbiology | 7 | 9% |
| Biochemistry, Genetics and Molecular Biology | 6 | 7% |
| Medicine and Dentistry | 5 | 6% |
| Other | 9 | 11% |
| Unknown | 22 | 27% |