| Title |
The HIV-1 Rev Protein Enhances Encapsidation of Unspliced and Spliced, RRE-Containing Lentiviral Vector RNA
|
|---|---|
| Published in |
PLOS ONE, November 2012
|
| DOI | 10.1371/journal.pone.0048688 |
| Pubmed ID | |
| Authors |
Bastian Grewe, Katrin Ehrhardt, Bianca Hoffmann, Maik Blissenbach, Sabine Brandt, Klaus Überla |
| Abstract |
During the RNA encapsidation process of human immunodeficiency virus (HIV) viral genomic, unspliced RNA (gRNA) is preferentially incorporated into assembling virions. However, a certain amount of spliced viral transcripts can also be detected in viral particles. Recently, we observed that nuclear export of HIV and lentiviral vector gRNA by Rev is required for efficient encapsidation. Since singly-spliced HIV transcripts also contain the Rev-response element (RRE), we investigated if the encapsidation efficiency of RRE-containing spliced HIV-vector transcripts is also increased by the viral Rev protein. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| France | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 3% |
| France | 1 | 3% |
| Germany | 1 | 3% |
| Unknown | 32 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 26% |
| Researcher | 4 | 11% |
| Student > Doctoral Student | 3 | 9% |
| Student > Master | 3 | 9% |
| Other | 2 | 6% |
| Other | 6 | 17% |
| Unknown | 8 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 31% |
| Agricultural and Biological Sciences | 8 | 23% |
| Immunology and Microbiology | 4 | 11% |
| Medicine and Dentistry | 3 | 9% |
| Social Sciences | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 8 | 23% |